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KMID : 0385219970070010057
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Korean Journal of Gerontology
1997 Volume.7 No. 1 p.57 ~ p.62
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Changes in the mRNA Expression of Mast Cell Specific Proteases in Tissues of Mice
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Kim Hyung-Min
Kim Chang-Young
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Abstract
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Mouse mast cell protease (MMCP)-2 mRNA was expressed by mast cells located in the mucosa of the stomach of WBB6F1-+/+ mice. Even in the stomach of WBB6F1-+/+ mice, mast cells located in the muscularis propria did not express MMCP-2 mRNA. The mRNA of MMCP-4 and mouse mast cell carboxypeptidase A were not expressed by mast cells in the stomach mucosa of non-treated WBB6F1-+/+ mice, but were expressed after the infection of Strongyloides venezuelensis. Cultured mast cells (CMCs) of WBB6F1-+/+ mice that expressed MMCP-2 mRNA were transplanted into the stomach wall of genetically mast cell-deficient WBB6F1-W/Wv mice. Mast cells that appeared in the mucosa expressed the MMCP-2 mRNA, but mast cells that appeared in the muscularis propria did not, indicating the adaptation of CMCs into a new environment. In contrast to CMCs, peritoneal mast cels (PMCs) of WBB6F1-+/+ mice that expressed MMCP-2 mRNA as well did not adapt to the muscularis propria of WBB6F1-W/Wv mice. The MMCP-2 mRNA remained to be expressed after the settlement in either the mucosa or the muscularis propria. Furthermore, the PMCs did not change the MMCP-4 and MMCP-6 mRNA expression phenotype after the settlement in either the mucosa or the muscularis propria of WBB6F1-W/Wv mice. These findings indicate that the dynamic changes in the mRNA expression of mast cell proteases may implicate their physiological importance.
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KEYWORD
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mouse mast cell protease, mouse mast cell carboxypeptidase A, mast cell-deficient mice, stomach mucosa, muscularis propria
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